1.What is the primary function of HPLC?
•A) Preliminary analysis
•B) Qualitative identification
•C) Quantification and purification
•D) TLC separation
•Answer: C) Quantification and purification
2.Which component of an HPLC system is responsible for pumping the mobile phase through the column?
•A) Detector
•B) Solvent reservoir
•C) High-pressure pump
•D) Injector system
•Answer: C) High-pressure pump
3.In reversed-phase HPLC, what is the nature of the stationary phase and the mobile phase, respectively?
•A) Polar stationary phase, polar solvent
•B) Polar stationary phase, non-polar solvent
•C) Non-polar stationary phase, polar solvent
•D) Non-polar stationary phase, non-polar solvent
•Answer: D) Non-polar stationary phase, non-polar solvent
4. Which application area commonly uses HPLC for drug stability testing?
•A) Environmental analysis
•B) Clinical tests
•C) Forensic tests
•D) Pharmaceuticals industry
•Answer:D) Pharmaceuticals industry
5.What is the principle behind size exclusion chromatography (SEC) in HPLC?
•A) Interaction with ionic groups
•B) Separation based on molecular size
•C) Reversed-phase mechanism
•D) Preliminary identification
•Answer: B) Separation based on molecular size
