RNA extraction, as shown in the image, involves multiple steps to… — Biotechnology Books & Pdf — TG.ME

RNA extraction, as shown in the image, involves multiple steps to isolate purified RNA from biological samples. The process includes the following key steps: 1. Cell Lysis • Lysis buffer is added to the sample (e.g., 30 mg of liver, gills, brain tissue). • The sample is homogenized and centrifuged to break open cells and release RNA. 2. Centrifugation • The mixture is centrifuged to separate cell debris, forming a pellet at the bottom. • The supernatant containing RNA is carefully transferred to a new tube. 3. Precipitation • 70% ethanol is added to the supernatant to help RNA precipitation. 4. RNA Binding • The solution is transferred to a spin column where RNA binds to the membrane inside. 5. Washing • A washing buffer is added to remove impurities. • The column is centrifuged, and the flow-through is discarded. 6. RNA Elution • RNase-free water is added to the column. • Another centrifugation step helps collect the purified RNA. 7. Final Purified RNA • The purified RNA is collected and ready for downstream applications like PCR, sequencing, and RNA interference studies. This process ensures high-quality RNA for further molecular biology experiments.